Cell & Immunobiology Core Facility
Providing the Best Tools... Achieving the Best Research

Flow Cytometry


Flow cytometry is a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of single cells flowing through an optical and/or electronic detection apparatus. By combining this with fluorescent tagging, vast amounts of data can be collected.


Beckman Coulter MoFlo XDP
 

The MoFlo™ XDP has set the standard for cell sorting with both functionality and speed. It is designed for researchers with features of improved productivity, an array of bio-safety features and easy-to-use software. This high-speed, configurable system has solid state lasers, high viability, high yield, an analysis rate of 100,000 events per second, and sort rates of up to 70,000 events per second. This allows the user to obtain high numbers of rare populations in a greatly reduced amount of time!

 
Analysis

*The following hourly rates will be billed in 30 minute increments:
 

  • With operator
  • $80 / hour
     
    Sorting
     
  • Set-up/shut-down fees
  • $100
  • With operator
  • $80 / hour
     
    Note: Only the operator can run this machine

    Beckman Coulter CyAn ADP
     

    The CyAn™ ADP High-Performance Flow Cytometer is a state-of-the-art flow cytometer that utilizes multiple laser excitation sources to analyze biological cells, beads, or other microscopic particles. The CyAn ADP has state-of-the-art optics, utilizes high performance digitization with pulse processing speed up to 70,000 events per second complementing a high acquisition and analysis capability of up to 100,000,000 events. Up to 9 x 9 channel compensation can be performed manually, using bi-exponential transformation (VisiComp) or automatically.

    Analysis

    *The following hourly rates will be billed in 30 minute increments:
     

  • Without operator
  • $50 / hour
  • With operator
  • $60 / hour


    BD FACScan
     

    The FACScan is a highly sensitive nonsorting instrument equipped with a 488nm-argon laser that is used exclusively for quantitative analysis applications. Since the 'Scan is equipped with three fluorescence detectors, simultaneous analysis of five parameters (two light scatter and three fluorochromes) is routine. FITC, PE, PI, and PerCP are the dyes most commonly used, but new dyes are continually being developed, expanding the range of this system. The FACScan is engineered with fixed optical, electronic and fluidic components, giving it the flexibility to function as an investigator-operated instrument after training.

    Analysis

    *The following hourly rates will be billed in 30 minute increments:
     

  • Without operator
  • $40 / hour
  • With operator
  • $60 / hour


    BD FACSCalibur
     

    The FACSCalibur is equipped with dual excitation lasers at the 488nm and 633nm wavelengths for quantitative analysis. The 'Calibur is also equipped with a sorting option for fragile cells unsuited for the stresses of high speed sorting. Since the 'Calibur is equipped with four fluorescence detectors, simultaneous analysis of six parameters (two light scatter and four fluorochromes) is routine. Possessing fixed optical, electrical, and fluidic components, this system can also be utilized without operator assistance after training.

     
    Analysis

    *The following hourly rates will be billed in 30 minute increments:
     

  • Without operator
  • $50 / hour
  • With operator
  • $70 / hour
     
    Sorting
     
  • Set-up/shut-down fees
  • $50
  • Without operator
  • $50 / hour
  • With operator
  • $70 / hour


    BD FACSDiVa'Vantage
     

    Our latest upgrades have made this instrument one of the most advanced, state of the art flow cytometers currently available. With three laser capability and very advanced optics, this system provides enormous flexibility in terms of applications as well as unprecedented sensitivity. The 'Vantage's multiple configurations makes it the instrument of choice for the development of new applications and for the analysis and sorting of highly complex populations of cells and cells with very low expression of the antigen of interest. This system is capable of up to 10 parameter/8 color high-speed sorting or analysis and is upgradeable to 16 parameter/14 color analysis or sorting. The 'Vantage has been upgraded with the Digital option ('DiVa) and high speed multi-laser sorts of up to four populations simultaneously are now being done. Not all experiments are suited for hi-speed sorting so cells that are too fragile: cells stimulated to become apoptotic, dendritic cells, and microglia, for example, are sorted under more gentle conditions with the 'Calibur.

     
    Analysis

    *The following hourly rates will be billed in 30 minute increments:
     

  • Use of 1-2 lasers
  • $80 / hour
  • Use of 3 lasers
  • $90 / hour
     
    Sorting
     
  • Set-up / shut-down fees
  • $100
  • Use of 1-2 lasers
  • $80 / hour
  • Use of 3 lasers
  • $90 / hour
     
    Note: Only the operator can run this machine


    Flow Links
     

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