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Biomolecular Research Facilities
University of Missouri-Columbia

Transgenic Animal Core (TAC)
Pronuclear Injection: Transgenic Mice
- Pronuclear microinjection sublicense agreement
- DNA preparation
- Environmental Health & Safety/Biosafety Committee
- Production of transgenic mice by pronuclear injection
- Selection of mouse strain
- PCR screening of trangenic offspring
- Health monitoring
- MU Animal Care and Use Committee

Embryonic Stem Cell Targeting
- Overview of services
- Investigator responsibilities
- Cell line selection
- Use of Gene Trap ES cell lines
- Construct design
- Electroporation and selection of ES cells
- Expansion of ES clones
- Blastocyst microinjection/embryo transfer
- Health monitoring/transfer of chimeric offspring

Service Request Procedures
- Procedures for establishing a project
- Patent/Sub-license Agreement
- Clients within the University of Missouri System
- Clients external to the University of Missouri System
- Submission Request Form - Transgenic projects
- Submission Request Form - Knockout projects
- MTA form for University of Missouri clients
- MTA form for external clients
Pronuclear Injection
Chimeric Offspring